Search results for " Molecular Confinement"
showing 2 items of 2 documents
Printing Biology for Advanced Synthetic Biosystem
2019
Printing technologies represent a powerful tool for the direct micro- and nano- fabrication of biomolecular structures at the interface between life and materials sciences (Arrabito et al., 2012). Their continuous development over the last years has permitted the onset of man-made biosystems with customizable dimensions (from the micron-scale down to the nanometer scale), composition (organic molecules, DNA, proteins, phospholipids), and relevant functions (molecular interactions, drug screening, cellular biointerfaces, cell-like compartments). In this work, we show the possibility to leverage the fabrication of a wide class of solid-supported or liquid-liquid based synthetic compartments b…
Molecular Confinement in Femtoliter scale aqueous Compartments
2017
Molecular confinement is known to lead to acceleration of molecular dynamics along with surface interaction. Nature employs confinement in molecularly crowded, heterogeneous and, specialized femtoliter (fL) compartments inside living cells for spontaneously achieving higher reaction efficiency and spatial-programming of composite, multi-step biochemical processes. We here show the facile production of aqueous fL droplets for studying molecular confinement on a biochip. We prepare fL aqueous droplets in oil drops on solid substrates by a “field-free”- no external electric fields and electrolytes - piezoelectric inkjet printing in which a novel actuating waveform is employed by picoliter size…